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The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted...
A peptide hydrogel for in vitro culture of chondrocytes was prepared and investigated based on the designed and synthesised peptide SGGAGGAGGAGGAGGS. Chondrocytes cultured in the peptide hydrogels were observed for changes of morphology characteristic, and the chondrocyte-seeded peptide hydrogels at the designed time point were obtained and the analysis of DNA content, histological staining and immuno-histochemistry...
Fau gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein FUBI at the N-terminus and ribosomal protein S30 at the C-terminus. Here we have cloned the cDNA and the genomic sequence of fau gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and Touchdown-PCR,...
The HSPB1' office full name is heat shock protein beta-1, which is well-defined as a stress-inducible protein. It is a significant and interesting work to study it . The cDNA and the genomic sequence of HSPB1 was cloned successfully from the Giant Panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively, Both sequences were sequenced and analyzed preliminarily. The cDNA...
RPL18A is a component of the 60S large ribosomal subunit encoded by RPL18A gene, which plays an important role in ribosome. To explore the structure characteristic of ribosomal protein L18 (RPL18A) gene of the Giant Panda (Ailuropoda melanoleuca) and the differences with other species reported,we have designed primers which based on the already-known information of RPL18A gene. Then, the cDNA and...
Ribosomal protein L11 (rpL11) which is a component of the 60s large ribosomal subunit plays an important role in ribosome. To explore the structure characteristic of rpL11 gene of the Ailuropoda melanoleuca, we have designed primers based on the known nucleotide sequence of rpL11 gene. The RT-PCR and PCR strategy were employed to isolate cDNA and genome of rpL11 from the Ailuropoda melanoleuca respectively,...
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