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In this work, two novel background correction (BC) methods, along with several commonly used ones, are evaluated regarding noise reduction in eleven two-channel self-versus-self (SVS) hybridizations. The evaluation of each BC method is investigated under the use of four statistical criteria combined into a single measure, the polygon area measure. Overall, our proposed BC approaches perform very well...
It has been shown previously that glucocorticoids exert a dual mechanism of action, meaning cytotoxic and mitogenic as well as mitogenic and anti-apoptotic, in a dose-dependent manner on CCRF-CEM cells at 72 h. Early gene expression response suggested also a dose-dependent dual mechanism of action of prednisolone which is apparently reflected on cell state upon 72 h of treatment. The present work...
The objective of the present study was the analysis of microarray data from a T-cell leukemia cell line (CCRF-CEM), treated with two different prednisolone concentrations, using four different pre-processing methods, within the Matlabreg computing environment. We have compared these methods using hierarchical clustering. The gene expression patterns revealed by hierarchical clustering were used to...
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