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A nanofluidic preconcentrator with the capability of rapidly preconcentrating and precisely positioning protein bands in multiple microchannels has been developed for highly sensitive detection of biomolecules. A novel electrical resistive network model is developed to guide the design of the nanofluidic preconcentrator which consists of a PDMS slab bonded with a glass slide. In the prototype design,...
This paper presents a micro-/nanofluidic biochip for enhanced sensitivity of immunoassay. By using a protein preconcentrator based on nanofluidic filters, we greatly improve the binding kinetics of immunoassay. A shallow nanofluidic channel connecting two microchannels for ion-selective filter is etched on the glass surface and sealed by PDMS/Glass irreversible bonding treated with O2 plasma. Our...
A simple method is proposed to fabricate channels with a depth in the nanometer range on a borosilicate glass substrate without cost-expensive lithography. Nanochannels are constructed with bulk micromachining by BOE wet etching process. Sub-60 nm deep nanofluidic channels on chip are formed after glass-glass fusion bonding, which are confirmed by using various methods of nanometer scale measurement...
A simple method is proposed to fabricate channels with a depth in the nanometer range on a borosilicate glass substrate without cost-expensive lithography. Nanochannels are constructed with bulk micromachining by BOE wet etching process. Sub-60 nm deep nanofluidic channels on chip are formed after glass-glass fusion bonding, which are confirmed by using various methods of nanometer scale measurement...
A simple method is proposed to fabricate channels with a depth in the nanometer range on a borosilicate glass substrate without cost-expensive lithography. Nanochannels are constructed with bulk micromachining by BOE wet etching process. Sub-60 nm deep nanofluidic channels on chip are formed after glass-glass fusion bonding, which are confirmed by using various methods of nanometer scale measurement...
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