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We have recently reported the lateral and rotational diffusion parameters for I-A k molecules expressing various cytoplasmic truncations (Int. Immunol. 12 (2000) 1319). We now describe the membrane dynamics of I-A k with various mutations in the presumed contact region between αβ-heterodimers in an (αβ) 2 dimer of dimers structure. Such mutations are known to strongly affect...
Fluorescence photobleaching recovery (FPR) measurements of cell surface protein lateral diffusion typically employ an interrogated spot of 0.5μm 1/e2 radius. The effective spot area represents only 1/500 of the total surface of an 8-μm cell. An FPR measurement of a protein expressed as 50,000 copies per cell reflects the dynamics of 100molecules. This limits the precision and reproducibility of FPR...
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