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Transcriptional activity of the gilthead sea bream (Sparus aurata) growth hormone (saGH) gene promoter has been investigated using transient transfection assays in GH3 cells. Analysis of two fragments (1.7 kb and a 5′-deleted 0.9 kb) of saGH gene promoter directed luciferase reporter gene activity, indicating transcriptional activity of both fragments in vitro. The shorter fragment, containing five...
The 5′ flanking region (1712 bp) of the growth hormone (GH) gene of the gilthead sea bream Sparus aurata was cloned, sequenced and characterized. It contains a consensus sequence for TATA box and several Pit-1 binding site motifs. Consensus sequences related to cyclic AMP response element, glucocortiocoid response element, and several other transcription factors were identified by comparison to consensus...
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