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A recombinant Saccharomyces cerevisiae strain over-expressing the fatty acid synthase of S. cerevisiae and the glucose dehydrogenase of Bacillus subtilis was applied for enantioselective reduction of ethyl 4-chloro acetoacetate (CAAE) to ethyl (S)-4-chloro-3-hydroxybutanoate (S-CHBE) as well as the reduction of ethyl benzoylacetate (EBA) to ethyl (S)-3-hydroxy-3-phenylpropionate (S-HPPE). The reaction...
We tried to establish a subculture system for cells from the Oka organ (lymphoid tissue) of the grass prawn Penaeus monodon. The basic culture medium was tested for osmolality, serum concentration, serum sources and pH. It was found that Leibovitz's L-15 medium supplemented with 10% fetal bovine serum, 5 g l -1 NaCl, pH 7.63-8.1, with final osmolality at 470-500 mmol kg -1...
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