Search results for: Claus Kerkhoff

Items from 1 to 19 out of 19 results

article

Targeting of NADPH oxidase in vitro and in vivo suppresses fibroblast activation and experimental skin fibrosis

Heba Dosoki, Agatha Stegemann, Muna Taha, Hans Schnittler, more

Experimental Dermatology > 26 > 1 > 73 - 81

Although there is increasing evidence that oxidative stress is involved in collagen synthesis and myofibroblast activation, the NADPH oxidase (Nox) system is incompletely investigated in the context of human dermal fibroblasts (HDFs) and skin fibrosis. Using the pan‐Nox inhibitor diphenyleneiodonium (DPI) as an initial tool, we show that gene expression of collagen type I, α‐smooth muscle actin (α‐SMA)...

article

Is Nox4 a key regulator of the activated state of fibroblasts in systemic sclerosis?

Markus Böhm, Heba Dosoki, Claus Kerkhoff

Experimental Dermatology > 23 > 9 > 679 - 681

The family of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases consists of phagocytic gp91^phox and six‐related isoforms. Recent evidence indicates that the NADPH oxidase isoform Nox4 controls vascular, renal and pulmonary injury. We propose that Nox4 is an intrinsic regulator of the activated state of dermal fibroblasts in systemic sclerosis (SSc). Profibrotic cytokines on the one hand...

article

Novel insights into the role of S100A8/A9 in skin biology

Claus Kerkhoff, Andreas Voss, Thomas E. Scholzen, Michelle M. Averill, more

Experimental Dermatology > 21 > 11 > 822 - 826

S100A8 and S100A9 belong to the damage‐associated molecular pattern molecules. They are upregulated in a number of inflammatory skin disorders. Owing to their abundance in myeloid cells, the main function of S100A8/A9 has been attributed to their role in inflammatory cells. However, it is becoming increasingly clear that they also exert important roles in epithelial cells. In this review, we discuss...

article

Double-stranded RNA induces S100 gene expression by a cycloheximide-sensitive factor

Andreas Voss, Kirsten Gescher, Andreas Hensel, Wolfgang Nacken, more

FEBS Letters > 2012 > 586 > 2 > 196-203

Viral double-stranded RNA (dsRNA) and its synthetic analog polyI:C are recognized via multiple pathways and induce the expression of genes related to inflammation. In the present study, we demonstrated the polyI:C-induced gene expression of the damage associated molecular pattern (DAMP) molecules S100A8 and S100A9, while other S100 genes were not affected. Cycloheximide and Brefeldin A treatment revealed...

article

Expression of S100A8/A9 in HaCaT keratinocytes alters the rate of cell proliferation and differentiation

Andreas Voss, Günther Bode, Claudia Sopalla, Malgorzata Benedyk, more

FEBS Letters > 2011 > 585 > 2 > 440-446

S100A8/A9 promotes NADPH oxidase in HaCaT keratinocytes and subsequently increases NFκB activation, which plays important roles in the balance between epidermal growth and differentiation. S100A8/A9-positive HaCaT cells present with a significantly reduced rate of cell division and greater expression of two keratinocyte differentiation markers, involucrin and filaggrin, than control cells. S100A8/A9...

article

S100A8/A9: A Janus-faced molecule in cancer therapy and tumorgenesis

Saeid Ghavami, Seth Chitayat, Mohammad Hashemi, Mehdi Eshraghi, more

European Journal of Pharmacology > 2009 > 625 > 1-3 > 73-83

Correlations exist between the abundance of S100 proteins and disease pathologies. Indeed, this is evidenced by the heterodimeric S100 protein complex S100A8/A9 which has been shown to be involved in inflammatory and neoplastic disorders. However, S100A8/A9 appears as a Janus-faced molecule in this context. On the one hand, it is a powerful apoptotic agent produced by immune cells, making it a very...

article

S100A8/9 induces cell death via a novel, RAGE-independent pathway that involves selective release of Smac/DIABLO and Omi/HtrA2

Saeid Ghavami, Claus Kerkhoff, Walter J. Chazin, Kamran Kadkhoda, more

BBA - Molecular Cell Research > 2008 > 1783 > 2 > 297-311

A complex of two S100 EF-hand calcium-binding proteins S100A8/A9 induces apoptosis in various cells, especially tumor cells. Using several cell lines, we have shown that S100A8/A9-induced cell death is not mediated by the receptor for advanced glycation endproducts (RAGE), a receptor previously demonstrated to engage S100 proteins. Investigation of cell lines either deficient in, or over-expressing...

article

The hetero-oligomeric complex of the S100A8/S100A9 protein is extremely protease resistant

Wolfgang Nacken, Claus Kerkhoff

FEBS Letters > 2007 > 581 > 26 > 5127-5130

S100A8, S100A9 and S100A12 proteins are associated with inflammation and tissue remodelling, both processes known to be associated with high protease activity. Here, we report that homo-oligomeric forms of S100A8 and S100A9 are readily degraded by proteases, but that the preferred hetero-oligomeric S100A8/A9 complex displays a high resistance even against proteinase K degradation. S100A12 is not as...

article

S100A9 deficiency alters adenosine-5′-triphosphate induced calcium signalling but does not generally interfere with calcium and zinc homeostasis in murine neutrophils

Wolfgang Nacken, Frank C. Mooren, Marie-Pierre Manitz, Günther Bode, more

International Journal of Biochemistry and Cell Biology > 2005 > 37 > 6 > 1241-1253

The two calcium- and zinc-binding proteins, S100A9 and S100 A8, abundant in myeloid cells are considered to play important roles in both calcium signalling and zinc homeostasis. Polymorphonuclear neutrophils from S100A9 ko mice are also devoid of S100A8. Therefore, S100A9-deficient neutrophils were used as a model to study the role of the two S100 proteins in the neutrophils's calcium and zinc metabolism...

article

L-FABP is exclusively expressed in alveolar macrophages within the myeloid lineage - Evidence for a PPARα-independent expression

Christian Schachtrup, Thomas E. Scholzen, Veronika Grau, Thomas A. Luger, more

International Journal of Biochemistry and Cell Biology > 2004 > 36 > 10 > 2042-2053

Peroxisome proliferator-activated receptors (PPARs) play a role in inflammation and, in particular, PPARγ is involved in monocyte/macrophage differentiation. Members of the fatty acid-binding protein (FABP) family have been reported to function as transactivators for PPARs. Therefore, the expression of PPARs and FABPs in the myeloid lineage was investigated by real-time PCR and immunofluorescence...

article

The myeloid expressed EF-hand proteins display a diverse pattern of lipid raft association

Wolfgang Nacken, Clemens Sorg, Claus Kerkhoff

FEBS Letters > 2004 > 572 > 1-3 > 289-293

EF-hand proteins are known to translocate to membranes, suggesting that they are involved in signaling events located in the cell membrane. Many proteins involved in signaling events associate cholesterol rich membrane domains, so called lipid rafts, which serve as platforms for controlled protein–protein interaction. Here, we demonstrate that the myeloid expressed EF-hand proteins can be distinguished...

article

Absence of S100A12 in mouse: implications for RAGE-S100A12 interaction

Georg Fuellen, Dirk Foell, Wolfgang Nacken, Clemens Sorg, more

Trends in Immunology > 2003 > 24 > 12 > 622-624

article

Interaction of S100A8/S100A9−Arachidonic Acid Complexes with the Scavenger Receptor CD36 May Facilitate Fatty Acid Uptake by Endothelial Cells ^†

Claus Kerkhoff, Clemens Sorg, Narendra N. Tandon, Wolfgang Nacken

Biochemistry > 2001 > 40 > 1 > 241 - 248

article

Zinc binding reverses the calcium-induced arachidonic acid-binding capacity of the S100A8/A9 protein complex

Claus Kerkhoff, Thomas Vogl, Wolfgang Nacken, Claudia Sopalla, more

FEBS Letters > 1999 > 460 > 1 > 134-138

Analysis of the calcium-induced arachidonic acid (AA) binding to S100A8/A9 revealed that maximal AA binding was achieved at molar ratios of 1 mol S100A8 and 1 mol S100A9 and for values greater than 3 calciums per EF-hand. The AA binding capacity was not induced by the binding of other bivalent cations, such as Zn ² ⁺ , Cu ² ⁺ , and Mg ² ⁺ , to the protein complex...

article

Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9)

Claus Kerkhoff, Martin Klempt, Clemens Sorg

BBA - Molecular Cell Research > 1998 > 1448 > 2 > 200-211

The two migration inhibitory factor- (MIF)-related protein-8 (MRP8; S100A8) and MRP14 (S100A9) are two calcium-binding proteins of the S100 family. These proteins are expressed during myeloid differentiation, are abundant in granulocytes and monocytes, and form a heterodimeric complex in a Ca ² ⁺ -dependent manner. Phagocytes expressing MRP8 and MRP14 belong to the early infiltrating cells...

article

Substrate Specificity of Acyl-CoA:Lysophospholipid Acyltransferase (LAT) from Pig Spleen

Claus Kerkhoff, Kai Habben, Lars Gehring, Klaus Resch, more

Archives of Biochemistry and Biophysics > 1998 > 351 > 2 > 220-226

The present investigation was undertaken to gain insights into the nature of both substrate binding sites of acyl-CoA:lysophospholipid acyltransferase (LAT) which could be potentially useful for the identification and purification of this specific acyltransferase. Therefore, we have investigated the specificity of LAT from crude membranes of pig spleen toward various 1-palmitoyl-glycerophospholipids...

article

The Mitogen-Induced Lysophospholipid:acyl-CoA Acyltransferase (LAT) Expression in Human T-Lymphocytes Is Diminished by Hydrocortisone

Claus Kerkhoff, Lars Gehring, Kai Habben, Klaus Resch, more

Biochemical and Biophysical Research Communications > 1997 > 237 > 3 > 632-638

One of the earliest changes observed in activated lymphocytes is the enhanced incorporation of unsaturated fatty acids into membrane phospholipids catalyzed by phospholipases and acyltransferases. This early membrane phospholipid remodeling has been shown to be independent from protein synthesis. We have investigated the oleic acid incorporation into phospholipids of activated T-lymphocytes within...

article

Acyl-CoA binding protein ( ACBP) regulates acyl-CoA: cholesterol acyltransferase ( ACAT) in human mononuclear phagocytes

Claus Kerkhoff, Martin Beuck, Jan Threige-Rasmussen, Friedrich Spener, more

Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism > 1997 > 1346 > 2 > 163-172

It is demonstrated that the acyl-CoA:cholesterol acyltransferase (ACAT) enzyme activity in rough endoplasmatic reticulum membranes is regulated by the acyl-CoA binding protein (ACBP). The ACAT activity is strongly inhibited by different ACBP/oleoyl-CoA complexes depending from the molar ratio of protein and fatty acid-CoA. Other lipid binding proteins such as bovine serum albumin and the liver fatty...

article

Identification of two different lysophosphatidylcholine:acyl-CoA acyltransferases (LAT) in pig spleen with putative distinct topological localization

Claus Kerkhoff, Lars Gehring, Kai Habben, Klaus Resch, more

Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism > 1996 > 1302 > 3 > 249-256

The lysophosphatidylcholine:acyl-CoA acyltransferase (LAT, EC 2.3.1.23) is an integral membrane protein participating in the membrane turnover and the T-cell activation process. Here, we present data that crude membranes of pig spleen contain two different LAT enzyme activities based on topological localization studies and the enzyme specificities towards various acyl-CoAs. When crude membranes...

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Keywords

INFLAMMATION (3)
ARACHIDONIC ACID (2)
FIBROSIS (2)
REACTIVE OXYGEN SPECIES (2)
RECONSTITUTION (2)
S100 PROTEIN (2)
SOLUBILIZATION (2)
STRESS RESPONSE (2)
CA 2 + (1)
CA 2 + BINDING PROTEIN (1)
CU 2 + (1)
ZN 2 + (1)
(PIG SPLEEN) (1)
1-ACYL-GLYCERO-3-PHOSPHOCHOLINE:ACYL-COA ACYLTRANSFERASE (1)
1-ACYL-GLYCERO-3-PHOSPHOLIPID (1)
1-PALMITOYL-GLYCERO-3-PHOSPHOCHOLINE (1)
ACYL-COA BINDING PROTEIN (1)
ACYL-COA SPECIFICITY (1)
ACYL-COA: CHOLESTEROL ACYLTRANSFERASE (1)
ADHESION (1)
APOPTOTIC AGENT (1)
ARTIFICIAL MEMBRANES (1)
ATP (1)
BCL2 PROTEIN FAMILY (1)
CALCIUM SIGNALLING (1)
CALCIUM-BINDING PROTEIN (1)
CANCER REGRESSION (1)
CELL DIFFERENTIATION (1)
CELL GROWTH (1)
CHEMOATTRACTANCE (1)
CHOLESTEROL ESTERIFICATION (1)
COLLAGEN (1)
CYTOKINE BURST (1)
CYTOSKELETON (1)
DERMAL FIBROBLASTS (1)
DIAZEPAM BINDING INHIBITOR PROTEIN (1)
DRP1 (1)
EF-HAND (1)
ENZYME INHIBITION (1)
EPITHELIAL CELL (1)
FATTY ACID BINDING PROTEIN (1)
FATTY ACID-BINDING PROTEIN (1)
FIBROBLASTS (1)
FUNCTIONAL HETEROGENEITY (1)
GENE REGULATION (1)
GRANULOCYTE (1)
GRANULOCYTES (1)
GROWTH ARREST (1)
IMMUNE ESCAPE (1)
IMMUNOFLUORESCENCE (1)
LIPID RAFT (1)
LYSOPHOSPHATIDYLCHOLINE:ACYL-COA ACYLTRANSFERASE (1)
METASTASIS (1)
MITOCHONDRIAL FISSION (1)
MONOCYTE/MACROPHAGE (1)
MYELOID DIFFERENTIATION (1)
MYOFIBROBLAST (1)
MYOFIBROBLASTS (1)
N-OCTYL GLUCOPYRANOSIDE (1)
NADPH (1)
NADPH OXIDASE (1)
NFΚB ACTIVATION (1)
NON-VIRAL DSRNA (1)
ORGANOTYPIC SKIN MODEL (1)
PERIPHERAL BENZODIAZEPINE BINDING SITE (1)
PIG SPLEEN (1)
POLYMORPHONUCLEAR LEUKOCYTE (1)
PROTEASE RESISTANCE (1)
PROTEIN STRUCTURE (1)
PROTEINASE K (1)
PSORIASIS (1)
REAL-TIME QUANTITATIVE PCR (1)
RECEPTOR FOR ADVANCED GLYCATED ENDPRODUCTS (RAGE) (1)
S100/CALGRANULIN (1)
S100/CALGRANULIN – RAGE AXIS (1)
S100A12 (1)
S100A8/A9 (1)
S100A9 (1)
SCLERODERMA (1)
SECONDARY RESPONSE GENE (1)
SECRETION (1)
SKIN BARRIER DYSFUNCTION (1)
SYSTEMIC SCLEROSIS (1)
TISSUE REPAIR (1)
TOPOLOGICAL LOCALIZATION (1)
TRANSFORMING GROWTH FACTOR‐Β (1)
TUMORGENESIS (1)
WOUND HEALING (1)
XIAP (1)
ZINC (1)
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Elsevier (15)
Wiley (3)
ACS (1)

Journal

FEBS Letters (5)
Experimental Dermatology (3)
BBA - Molecular Cell Research (2)
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism (2)
International Journal of Biochemistry and Cell Biology (2)
Archives of Biochemistry and Biophysics (1)
Biochemical and Biophysical Research Communications (1)
Biochemistry (1)
European Journal of Pharmacology (1)
Trends in Immunology (1)
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Search results for: Claus Kerkhoff

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