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Mammalian cells contain suppressor tRNAs that can translate nonsense codons such as UAG and UGA localized at a specific site of natural mRNAs. For translation of these nonsense codons, a specific secondary or tertiary structure of mRNAs located in the region surrounding the translatable nonsense codon is required. In mammalian cells, transcriptional expression of the tRNA gene encoding UAG suppressor...
Pyrodictium occultum is a hyperthermophilic archaeum that grows optimally at 105 o C. To study how tRNA molecules in P occultum are thermally stabilized, we isolated the initiator tRNA gene from the organism using a synthetic DNA probe of 74 bp containing the known nucleotide sequences that are conserved in archaeal initiator tRNAs. A HindIII fragment of 700 bp containing thePyrodictium...
Two rat osteosarcoma cell lines, YROS-1 and YROS-2, were established from two experimental osteosarcomas and induced by internal irradiation with radioactive phosphorus. Both cell lines formed a monolayer cell sheet in vitro with focal piling. The YROS-1 cells were re fractile and spindle or polygonal in shape, whereas the YROS-2 cells were flat, spread and polygonal in shape. Ultrastructurally, the...
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