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Constitutive isoforms of nitric oxide synthase (NOS) are activated by transient binding of Ca 2+ /Calmodulin. Here, we characterize the binding of Calmodulin to purified neuronal NOS (nNOS). [ 125 I]Calmodulin bound to a single class of non-interacting and high affinity sites on nNOS. [ 125 I]Calmodulin binding achieved rapid saturation, was linear...
Nitrogen oxide-containing compounds displaced the peripheral benzodiazepine ligand [ 3 H]Ro5-4864 from guinea pig membrane preparations. Sodium nitroprusside (SNP) was the most potent (IC 50 = 5.61 +/- 1.72 x 10 -5 M). Moreover, its ability to bind to these receptors showed marked tissue variability (heart > kidney cerebral cortex). When tested on rat atrium,...
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