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The present study was performed to determine whether the expression levels of the hypothalamic cholecystokinin (CCK) and its receptors are associated with the responsiveness to high frequency electroacupuncture (EA) analgesia in rats. EA stimulation (100Hz, 0.5ms pulse width, 0.2–0.3mA) was delivered to the Zusanli (ST36) acupoint of male Sprague–Dawley rats for 20min without anesthetics or holder...