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The preparation of pure iso-α-acids from hop-derived extracts is described. The method relies on the chemical separation of cis- and trans-isomers of the iso-α-acids, by the precipitation of the trans-isomers with dicyclohexylamine (DCHA), followed by C 18 reversed-phase preparative HPLC using a mobile phase of ethanol-water-glacial acetic acid (60:40:0.2, v/v). The selectivity of ethanol...