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The complete sequence of a Singapore isolate of odontoglossum ringspot virus (ORSV-S1) comprises 6609 nucleotides (nt) and four open reading frames (ORFs 1 to 4). The 126-kDa RNA-dependent RNA polymerase (RdRp), 33-kDa movement protein (MP) and 18-kDa coat protein (CP) cistrons are located at nt 63-3401, 4807-5718, and 5721-6197 on the genome, respectively. The 5 UTR contains three copies of an...
A rapid and flexible propagation system for Heliconia psittacorum was developed using thin sections (1 mm) of the base of in vitro plantlets. When cultured on Murashige and Skoog medium in the presence of 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) the ex-plants produced rooted plantlets. Forty to 80 μM 2,4-D induced the formation of highly morphogenic callus. When subcultured on basal medium,...
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