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This study describes a method for the quantification of active ligand surface concentration for bulk-modified hydrogels. Two poly(propylene fumarate-co-ethylene glycol) (P(PF-co-EG)) block copolymers were synthesized with terminal poly(ethylene glycol) (PEG) chains of number average molecular weight 1960 and 5190g/mol. Hydrogels were synthesized with bulk-modified biotin as a model ligand, making...
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