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This study examined the effect of three extenders, Marine Fish Ringer (MFR), sodium citrate (CT), and sodium chloride (NaCl) and two cryoprotectants (10% dimethyl sulfoxide—DMSO and 15% Trehalose) on the cryopreservation of giant grouper, Epinephelus lanceolatus sperm which was later used for hybridization trial with orange-spotted grouper, Epinephelus coioides. Fish Ringer and sodium citrate as extenders...