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We have determined the relationship between microtubule length and translocation velocity from recordings of bovine brain microtubules translocating over a Paramecium 22S dynein substratum in an in vitro assay chamber. For comparison with untreated samples, the 22S dynein has been subjected to detergent and/or to pretreatments that induce phosphorylation of an associated 29 kDa light chain. Control...
Eukaryotic cells have many proteins that cap the barbed ends of actin filaments. Manipulation of their cellular concentration leads to changes in cell motility rates, actin dynamics and signal transduction reactions.
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