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We established a tetracycline-regulated gene expression system that tightly controls expression of genes in Dictyostelium discoideum. The control elements are contained in two plasmid vectors, one being an integrated plasmid encoding a chimeric tetracycline-controlled transcriptional activator protein (tTA s* ). The second component is an extrachromosomal plasmid harboring the gene of...
The outer membrane phospholipase A (OMPLA) of Escherichia coli is one of the few integral outer membrane proteins displaying enzymatic activity. It is encoded as a mature protein of 269 amino acids preceded by a signal sequence of 20 amino acids. There is no sequence homology with water-soluble lipases and phospholipases. Crystals of the mature enzyme were obtained at 22°C from 24-28% (v/v) 2-methyl-2,4-pentanediol...
Single crystals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 o C in 2-3 months. They belong to the orthorhombic space group P2 1 2 1 2 1 with a = 73.31 Å, b = 77.96 Å, and c = 169.81 Å, with two protein molecules per asymmetrical unit. The crystals...
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