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During hypothermic preservation of cells (0–4°C), metabolism is diminished and energy-dependent transport processes are arrested. The effect of hypothermic preservation of hepatocytes in endocytic transport following rewarming has not been previously reported. We evaluated the uptake of EGF (Epidermal Growth Factor) ligand conjugated to fluorescent Quantum Dots (QDs) probes in rat hepatocytes after...
During cell and tissue hypothermic preservation (HP) at 4°C, metabolism decelerates and energy-dependent processes such as endocytosis are arrested. Cold induced injury expressed after rewarming to normothermia, accelerates loss of membrane integrity and cell death. Receptor-mediated endocytosis is a temperature and energy dependent mechanism requiring intact membranes as well as the integrity of...
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