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The protolytic equilibrium of fluorescein in aqueous solutions was studied in the presence of cycloheptaamylose (β-cyclodextrin, or β-CD). The constants of stepwise ionization of the dye (H3R+⇆H2R⇆HR-⇆R2-), K a0 , K a1 , and K a2 were determined using vis-spectroscopy at ionic strength 0.05M (NaCl+buffer) and 25°C. In the presence of 0.0086M β-CD, the indices of ionization...