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Two model substrates, cellulose (particle size 20 μm) and powdered maize starch, were incubated in vitro in glass syringes with rumen fluid and buffer. The fermentation was monitored after 1 h and every 3 h, up to 24 h of incubation. At the end of each incubation period, gas yield was read and the contents of three syringes were removed, separated and analysed. Bacterial dry matter was calculated...