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Castilla-León introduced newborn screening (NBS) for Cystic Fibrosis (CF) in March 1999, being the first spanish region doing it. The screening consisted of an immunoreactive trypsinogen assay from dried blood (kit AutoDelfia Neonatal IRT (Perkin-Elmer) followed for analysis of more than the 80% of the CF causing mutations in our population. Samples with an IRT level higher than 70 ng/mL (cut-off)...