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An assay was developed for phosphofructokinase-1 (PFK-1) using capillary electrophoresis (CE). In the glycolytic pathway, this enzyme catalyzes the rate-limiting step from fructose-6-phosphate and magnesium-bound adenosine triphosphate (Mg–ATP) to fructose-1,6-bisphosphate and magnesium-bound adenosine diphosphate (Mg–ADP). This enzyme has recently become a research target because of the importance...