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Aims: To determine whether Clostridium botulinum neurotoxin (BoNT) production in anaerobic culture was affected by temperature and could influence the sandwich ELISA (sELISA) detection of group III toxins in pre‐enriched gastrointestinal (GI) contents from clinically suspect cattle botulism cases.
Methods and Results: Bovine post‐mortem GI samples taken from 124 and 96 animals with suspect and nonsuspect...
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