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Phosphoenolpyruvate carboxylase (PEPC) characterized by a specific activity of at least 40 units/mg; and/or by no more than 50% loss of activity after 60 days in 167 mM Tricine/HCl buffer, pH 8.0, and 15 mM MgCl2; and/or, at pH 6.5, an activity of at least 40% relative to the activity at optimum pH is disclosed. Such enzymatic activity can be obtained from serine pathway methylotrophs such as Hyphomicrobium,...
A formulation consisting essentially of -1,4-xylanase and -1,3-xylosidase, but essentially free of -1,4-glucanase and -1,4-cellobiohydrolase and on or more lactic acid-producing bacteria is disclosed. The formulation can be used in a silage composition to stabilize silage from cereal and other crops and to enhance its nutritive value and digestibility in a ruminant or other animal.