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The methylation status of ribosomal gene (rRNA) clusters have been investigated in a large variety of angiosperm species. Here we have analysed methylation in ribosomal gene (rRNA) clusters using MspI, HpaII, BstNI, EcoRII and CfoI restriction enzymes in combination with Southern hybridization to the 25S rDNA probe. It was shown that cytosine methylation at CpG dinucleotides and CpNpG trinucleotides...
Telomerase is a ribonucleoprotein enzyme which elongates the G-rich strand of telomeric DNA to compensate for the progressive reduction in its length due to incomplete replication of chromosome ends, which in human somatic cells leads to cell cycle arrest upon shortening of telomeres to a critical length. To examine the possible involvement of telomerase in metabolism of plant genetic material, we...
PCR and primers derived from the telomeric repeat (CCCTAAA) n and from the tobacco subtelomeric tandemly repetitive sequence HRS60 (EMBL X12489) were used to amplify the region linking the two loci. A 131 bp PCR product was obtained both from total tobacco DNA and from the DNA fraction enriched for telomeres. Its sequence only consists of the telomeric primer and the attached region of the...
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