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Small molecules identified through high-throughput screens are an essential element in pharmaceutical discovery programs. It is now recognized that a substantial fraction of small molecules exhibit aggregating behavior leading to false positive results in many screening assays, typically due to nonspecific attachment to target proteins. Therefore, the ability to efficiently identify compounds within...
A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (K m and V max ) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLC Bc ). The assay is applied to the determination of the K i for a known inhibitor of PLC Bc ...
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