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In the current work, we study the use of electrodeposited calcium alginate hydrogels in the development of enzymatic biosensors. Alginate electrodeposition involves the rupture of CaCO3 particles by protons from water electrolysis into Ca2+ and HCO3−. The released calcium ions cross-link alginate forming the hydrogel. Horseradish peroxidase (HRP, used as model enzyme) was electrodeposited on screen...