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The optimisation of a flow cytometric protocol for the determination of the estrogen receptor β (ERβ) expression in bovine blood neutrophils is described. The following final incubation conditions were obtained: fixation with 0.25% formaldehyde and 70% methanol, both for 1 h; permeabilisation with 0.05% Triton X-100, overnight labelling at 4 °C with the primary antibody diluted at 10 μg/ml and subsequent...
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