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Rat pancreatic islet homogenates display protein kinase C activity. This phospholipid-dependent and calcium-sensitive enzyme is activated by diacylglycerol or the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). In the presence of TPA, the K a for Ca 2+ is close to 5 μM. TPA does not affect phosphoinositide turnover but stimulates [ 32 P]-...