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Using Secondary Ion Microscopy and Spectrometry (SIMS), we have studied the distribution of K + , Na + , Mg 2+ and Ca 2+ cations in pollen grains of white birch (Betula verrucosa Ehrh.). To prevent ion loss during fixation, dehydration and embedding treatments, we developed a new preparation method (Verdus M.C. et al., in preparation.). Fixation and dehydration...