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The paper summarizes some of our recent findings in structure-function organization of the alcohol oxidase (AO) from two species of methylotrophic yeasts. As revealed by PAGE, in methanol-grown Hansenula polymorpha DL1 and Pichia methanolica MH4 along with AO, the non-flavin minor protein having AO activity and different type of subunits is expressed. HLPC analysis of the content of FAD and its modified...