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Fusion proteins were constructed between the porcine α 2A -adrenoceptor and either wild-type (Cys 351 ) or a pertussis toxin-resistant (Gly 351 ) form of the G protein G i1 α. Addition of adrenaline to membranes expressing the fusion proteins resulted in concentration-dependent stimulation of their high affinity GTPase activity. The α ...
Neuroblastoma glioma hybrid, NG108-15 cells were stably transfected with an epitope tagged variant of G s α (HA-G s α). The introduced HA-G s α was able to interact with the IP prostanoid receptor and was able to stimulate adenylyl cyclase activity as measured by an enhanced capacity of membrane extracts to reconstitute NaF-dependent adenylyl cyclase activity to membranes...
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