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An experiment was conducted to evaluate the ability of betaine to spare the choline requirement in juvenile tilapia. The basal diet contained 320 g crude protein/kg diet (101 g crude protein from casein and gelatin, and 219 g from a crystalline l-amino acid mixture). The total sulfur amino acid (TSAA) concentration of the basal diet was 5.0 g TSAA/kg diet, met:cys 89:11, the choline concentration...