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The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure...
We theoretically and experimentally demonstrated that direct backward third harmonic generation (THG) waves can be comparable in magnitude with forward THG waves in nanostructures, such as ZnO thin films and nanoparticles of CdSe and Fe3O4.
We demonstrated that the spectroscopic enhancement of third harmonic generation (THG) of human oxyhemoglobin and deoxyhemoglobin in the 1200–1300 nm region agreed with the absorption spectra and was dominated by three-photon/two-photon resonance.
We demonstrate molecular-specific third-harmonic-generation microscopy in cultured Hela cells and hamster oral cavity by using CdSe/ZnS/lipid quantum dots as contrast agent.
Through molecular-resonant third-harmonic-generation (THG) in hemoglobin, in vivo molecular THG microscopy of erythrocytes can be realized without using fluorescence and exogenous contrast agents. Studies in live hamster oral cavity indicate its superiority to image angiogenesis.
Ex vivo and in vivo oral cancer diagnoses are demonstrated using noninvasive third-harmonic-generation biopsy. Through its superior spatial resolution on nucleus, oral cancer cells in hamster oral cavities are successfully identified without any exogenous markers.
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