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A novel analytical workflow is presented for the analysis of time-dependent 13C-labeling of the metabolites in the methylotrophic bacterium Methylobacterium extorquens AM1 using gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Using 13C-methanol as the substrate in a time course experiment, the method provides an accurate determination of the number of carbons converted to the stable...