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The nucleotide (nt) sequence of the human cDNA encoding PAP, a pancreatic secretory protein induced during acute pancreatitis, was found to be identical with that of a gene activated in human primary hepatocellular cancer, designatedHIP . To obtain insight into the expression of PAP/HIP, we characterized the gene organization, especially focusing on the 5 -flanking region, and found that it spans...
rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided...
reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic...
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