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GNA-1 and GNA-2 are two G protein α subunits from the filamentous fungusNeurospora crassa.Loss ofgna-1leads to multiple phenotypes, while Δgna-2strains do not exhibit visible defects. However, Δgna-1Δgna-2mutants are more affected in Δgna-1phenotypes. Here we report a biochemical investigation of the roles of GNA-1 and GNA-2 in cAMP metabolism. Assays of Mg 2+ ATP-dependent adenylyl cyclase...