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SeveralChlorellavirus CVK2 proteins had chitosanase and/or chitinase activities. A gene coding for an ORF of 328 amino acids (aa) with a predicted molecular mass of 36,769 Da was cloned from the viral genome. The predicted amino acid sequence of an N′-portion (174 aa) of this gene product (vChta-1) showed 22 to 25% identity with various bacterial chitosanases. A glutathioneS-transferase (GST)–vChta-1...
Chlorellavirus CVK2 particles contain several capsid proteins that are selectively released by treatment with 4Murea. Seven of them (Vp52, Vp45, Vp41, Vp25, Vp20.5, Vp20, and Vp16.2) have been identified based on amino acid sequences at the N-terminus. Comparison of the amino acid sequences with the corresponding ORFs deduced from the nucleotide sequence of PBCV-1, the prototype member ofPhycodnaviridae,revealed...
Several capsid proteins were selectively released from the viral core ofChlorellavirus CVK2 by treatment with 4M urea. Among the viral core proteins, seven species (Vp154, Vp73, Vp63, Vp52, Vp48, Vp42, and Vp25) were shown to have DNA-binding activities by Southwestern blot analysis. Except for Vp154 and Vp25, these DNA-binding proteins showed a specific affinity for the viral genomic DNA. The viral...
Large (30–45 kbp) deletions were induced in theChlorellavirus CVK1 genome by UV irradiation. Restriction endonuclease maps of the mutant genomes showed that these deletions occurred in a region located from 1.5 kbp to 47 kbp from the left DNA end. The nucleotide sequences determined around the deletion boundaries indicate that the deletion process took place by both homologous and nonhomologous recombinations...