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To evaluate perfringolysin O, a cholesterol-dependent pore-forming cytolysin, as a tool to study several aspects of human sperm physiology.Prospective study.Basic research laboratory.Human semen samples with normal parameters obtained from healthy donors.Interaction of recombinant perfringolysin O with human spermatozoa.Assessment of perfringolysin O binding to spermatozoa, tests for acrosome and...