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When the thymine of T 4 DNA is replaced by 5-BU the melting temperature of T 4 DNA is increased from about 83° to about 93°C. Heating and slow cooling of T 4 DNA at concentrations of about 30μg/ml leads to aggregates which consist of several polynucleotide chains which appear in the electron microscope as a branched structure. The aggregates have regions which are true hybrids...