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An in vitro co-culture model of SCp2 mammary epithelial cells and Escherichia coli strains was established in bacterial non-CO2 incubators. Co-culturing SCp2 cells with either the rod-shaped W3110 or spherical-shaped GC7378Tn10 strains of Escherichia coli led to an increase in interleukin-6 (IL-6) levels by SCp2 cells after 9 h. At a ratio of 1:100 (epithelial:bacterial), the rod-shaped W3110 strain...
A multicopy clone was isolated which conferred resistance to the SOS inducer nitrofurantoin in an Escherichia coli lon mutant. Plasmid pHL1 was found to contain a 7–8 kbp HindIII DNA insert from a region of the chromosome at 88.5 minutes. Further characterisation of pHL1 revealed that resistance to nitrofurantoin was due to the overexpression of the hslV-hslU operon which encodes an ATP-dependent...
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