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Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%.
Cultured mesophyll protoplasts can be directly induced to follow different developmental pathways. In a medium containing auxins only, cells elongate and finally differentiate into very long tubular cells. NAA at a concentration of 1 mg/l was found to be the optimal trigger for this response. If both auxins and cytokinins are present in the culture medium, cell division is consequently induced...
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