Search results for: Alexandre Juillerat

Items from 1 to 16 out of 16 results

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Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV‐1 Infection

Marianna Romito, Alexandre Juillerat, Yik Lim Kok, Markus Hildenbeutel, more

Biotechnology Journal > 16 > 1 > n/a - n/a

Therapies to treat patients infected with human immunodeficiency virus (HIV) aim at preventing viral replication but fail to eliminate the virus. Although transplantation of allogeneic CCR5Δ32 homozygous stem cell grafts provided a cure for a few patients, this approach is not considered a general therapeutic strategy because of potential side effects. Conversely, gene editing to disrupt the C‐C chemokine receptor type 5 (CCR5)...

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Modulation of chimeric antigen receptor surface expression by a small molecule switch

Alexandre Juillerat, Diane Tkach, Brian W. Busser, Sonal Temburni, more

BMC Biotechnology > 2019 > 19 > 1 > 1-9

Background Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR) T-cells are designed to detect and kill tumor cells that present a specific, predefined antigen. The rapid expansion of targeted antigen beyond CD19, has highlighted new challenges,...

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Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin

Micheline Guillotte, Farida Nato, Alexandre Juillerat, Audrey Hessel, more

Malaria Journal > 2016 > 15 > 1 > 1-16

Background Rosetting, namely the capacity of the Plasmodium falciparum-infected red blood cells to bind uninfected RBCs, is commonly observed in African children with severe malaria. Rosetting results from specific interactions between a subset of variant P. falciparum erythrocyte membrane protein 1 (PfEMP1) adhesins encoded by var genes, serum components and RBC receptors. Rosette formation is a...

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Efficient strategies for TALEN-mediated genome editing in mammalian cell lines

Julien Valton, Jean-Pierre Cabaniols, Romàn Galetto, Fabien Delacote, more

Methods > 2014 > 69 > 2 > 151-170

TALEN is one of the most widely used tools in the field of genome editing. It enables gene integration and gene inactivation in a highly efficient and specific fashion. Although very attractive, the apparent simplicity and high success rate of TALEN could be misleading for novices in the field of gene editing. Depending on the application, specific TALEN designs, activity assessments and screening...

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Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

William Haun, Andrew Coffman, Benjamin M. Clasen, Zachary L. Demorest, more

Plant Biotechnology Journal > 12 > 7 > 934 - 940

Soybean oil is high in polyunsaturated fats and is often partially hydrogenated to increase its shelf life and improve oxidative stability. The trans‐fatty acids produced through hydrogenation pose a health threat. Soybean lines that are low in polyunsaturated fats were generated by introducing mutations in two fatty acid desaturase 2 genes (FAD2‐1A and FAD2‐1B), which in the seed convert the monounsaturated...

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Functional and immunological characterization of the var2CSA-DBL5ɛ domain of a placental Plasmodium falciparum isolate

Stéphane Gangnard, Nicaise G. Tuikue Ndam, Sedami Gnidehou, Michael Quiviger, more

Molecular & Biochemical Parasitology > 2010 > 173 > 2 > 115-122

Pregnancy-associated malaria (PAM) arises from sequestration of Plasmodium falciparum-parasitized erythrocytes (PE) in the placenta, leading to chronic symptoms in the expectant mother and serious consequences for fetal development. Placental sequestration has been linked to binding of chondroitin sulphate A (CSA) by the var2CSA variant of PfEMP1 expressed on the PE surface, and a substantial body...

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Biochemical and biophysical characterisation of DBL1α1-varO, the rosetting domain of PfEMP1 from the VarO line of Plasmodium falciparum

Alexandre Juillerat, Sébastien Igonet, Inès Vigan-Womas, Micheline Guillotte, more

Molecular & Biochemical Parasitology > 2010 > 170 > 2 > 84-92

Rosetting of erythrocytes infected with Plasmodium falciparum is frequently observed in children with severe malaria. This adhesion phenomenon has been linked to the DBL1α domain of P. falciparum erythrocyte membrane protein 1 (PfEMP1) in three laboratory clones: FCR3S1.2, IT4R29 and Palo Alto varO. Here, we compare the soluble recombinant NTS-DBL1α ₁ -varO domain (NTS: N-terminal segment)...

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A split-protein sensor for studying protein–protein interaction in mycobacteria

Helen O'Hare, Alexandre Juillerat, Petronela Dianišková, Kai Johnsson

Journal of Microbiological Methods > 2008 > 73 > 2 > 79-84

The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a split-protein sensor used to detect protein–protein interactions in tryptophan auxotrophic Saccharomyces...

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An Engineered Protein Tag for Multiprotein Labeling in Living Cells

Arnaud Gautier, Alexandre Juillerat, Christian Heinis, Ivan Reis Corrêa, more

Chemistry & Biology > 2008 > 15 > 2 > 128-136

The visualization of complex cellular processes involving multiple proteins requires the use of spectroscopically distinguishable fluorescent reporters. We have previously introduced the SNAP-tag as a general tool for the specific labeling of SNAP-tag fusion proteins in living cells. The SNAP-tag is derived from the human DNA repair protein O ⁶ -alkylguanine-DNA alkyltransferase (AGT) and...

article

Lipid Deposition on the Tongue after Oral Processing of Medium-Chain Triglycerides and Impact on the Perception of Mouthfeel

Urska Pivk, Nicolas Godinot, Coralie Keller, Nicolas Antille, more

Journal of Agricultural and Food Chemistry > 2008 > 56 > 3 > 1058 - 1064

article

Assessing Lipid Coating of the Human Oral Cavity after Ingestion of Fatty Foods

Urška Pivk, Nataša Poklar Ulrih, Marcel-Alexandre Juillerat, Peter Raspor

Journal of Agricultural and Food Chemistry > 2008 > 56 > 2 > 507 - 511

article

Structured fiber supports for gas phase biocatalysis

Takoua Debeche, Cynthia Marmet, Lioubov Kiwi-Minsker, Albert Renken, more

Enzyme and Microbial Technology > 2005 > 36 > 7 > 911-916

Pseudomonas cepaciae lipase adsorbed onto non-porous structured fiber supports in the form of woven fabrics, was used to catalyze hydrolysis and transesterification reactions in the gas phase. The enzyme adsorbed onto carbon fiber support exhibited much higher catalytic activity compared to the enzyme immobilized onto glass fiber carrier. The effect of temperature and relative humidity on reactions...

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Chemoenzymatic Synthesis of Aroma Active 5,6-Dihydro- and Tetrahydropyrazines from Aliphatic Acyloins Produced by Baker's Yeast

Toshinari Kurniadi, Rachid Bel Rhlid, Laurent-Bernard Fay, Marcel-Alexandre Juillerat, more

Journal of Agricultural and Food Chemistry > 2003 > 51 > 10 > 3103 - 3107

article

Directed Evolution of O ⁶ -Alkylguanine-DNA Alkyltransferase for Efficient Labeling of Fusion Proteins with Small Molecules In Vivo

Alexandre Juillerat, Thomas Gronemeyer, Antje Keppler, Susanne Gendreizig, more

Chemistry & Biology > 2003 > 10 > 4 > 313-317

We report here the generation of mutants of the human O ⁶ -alkylguanine-DNA alkyltransferase (hAGT) for the efficient in vivo labeling of fusion proteins with synthetic reporter molecules. Libraries of hAGT were displayed on phage, and mutants capable of efficiently reacting with the inhibitor O ⁶ -benzylguanine were selected based on their ability to irreversibly transfer the benzyl...

article

Biogeneration of 2-(1-hydroxyethyl)-4,5-dihydrothiazole as precursor of roasted and popcorn-like aroma for bakery products

Yvette Fleury Rey, Rachid Bel-Rhlid, Marcel-Alexandre Juillerat

Journal of Molecular Catalysis. B, Enzymatic > 2002 > 19-20 > Complete > 473-477

Bread aroma is one of the most important criteria of the quality of bakery product. Molecules exhibiting roasted notes largely contribute to this flavour and 2-acetyl-2-thiazoline (2-AT) was identified as one of the strongest. We report here the formation of its precursor 2-(1-hydroxyethyl)-4,5-dihydrothiazole via the bioconversion of cysteamine, ethyl-l-lactate and d-glucose with baker's yeast as...

article

Tomato ( Lycopersicon esculentum ) Pectin Methylesterase and Polygalacturonase Behaviors Regarding Heat- and Pressure-Induced Inactivation

Simon Crelier, Marie-Claude Robert, Johanna Claude, Marcel-Alexandre Juillerat

Journal of Agricultural and Food Chemistry > 2001 > 49 > 11 > 5566 - 5575

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