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Azotobacter vinelandii is a soil γ-proteobacteria that fixes nitrogen and forms desiccation-resistant cysts. The exopolysaccharide alginate is an integral part of the layers surrounding the cysts. Here, we reported the cloning of A. vinelandii algC, encoding the enzyme catalyzing the second step of alginate pathway. We showed that AlgC is involved not only in alginate production, but also in lipopolysaccharide...
Various long-chain choline derivatives, differing in the length of the hydrocarbon backbone, have been synthesized. The influence of these compounds on the activity of firefly luciferase has been studied. Whereas C 4 -C 10 derivatives do not influence enzyme activity, C 12 -C 18 derivatives inactivate luciferase rapidly and irreversibly. It is suggested...
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