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CD studies have shown that five tryptophan to phenylalanine (W->F) mutants of eukaryotic initiation factor-4E (eIF-4E) contain low amounts of α-helix, the main elements of secondary structure being β-sheets/turns and aperiodic regions. Interactions with the cap analog m 7 GpppG are accompanied by changes in overall secondary structure which include reductions, and in one case an increase...
Troponin C from turkey skeletal muscle has been compared with its chicken counterpart in terms of amino acid composition and fragmentation patterns and with rabbit TN-C by Ca 2+ binding and conformational response to Ca 2+ as monitored by CD and fluorescence. Cyanogen bromide and tryptic digestion mixtures of chicken and turkey TN-C have been separated by reversed-phase HPLC...
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