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The (CGG) repeats associated with X-chromosome fragility are generally believed to form quadruplexes. This notion has persisted although it had been shown that only very short (CGG) n sequences form quadruplexes and that this quadruplex formation occurs in conditions far from physiological. We have now studied, using CD and absorption spectroscopies, quadruplex formation of (CGG) n ...
We have used CD spectroscopy, polyacrylamide gel electrophoresis, and UV absorption spectroscopy to study conformational properties of DNA fragments containing (CCA) n and (TGG) n repeats, which are the most length-polymorphic microsatellite sequences of the human genome. The (CCA) n fragments are random single strands at neutral and alkaline pH but they fold into intramolecular...
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