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A sensitive, highly specific immunoassay method has been developed by measuring the enhanced resonance light scattering (RLS) signals of immunoreactions with simultaneously scanning both the excitation and the emission monochromators of a common spectrofluorometer. For a given content of antibody (Ab), the RLS signals of an immunoreaction follow Gaussian distribution with antigen (Ag) concentration...
An optical immunosensor based on measuring the surface enhanced light scattering (SELS) signals is developed by simultaneously scanning the excitation and the emission monochromators of a common spectrofluorometer. The formation of silanization on solid surface of glass slides and the binding of antibody with antigen in series have been confirmed by measuring the SELS signals. When 100μgml −1...
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