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Binding to estrogen and aryl hydrocarbon receptors (ER and AhR) is a possible mechanism for a compound to mediate endocrine-disrupting effects. In the present study, substances contained in sediment organic fractions from the Detroit River were screened for their ability to bind ER and/or AhR. Two in vitro bioassays were used: MELN cells, consisting of human breast cancer cells (MCF-7) stably transfected...