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An androst-4-ene-3,17-dione (AD)-producing mutant, AD1 and an androsta-1,4-diene-3,17-dione (ADD)-producing mutant, PD3 were derived from a cholesterol-, phytosterol-assimilating bacterium Mycobacterium sp. B-3805S and a cholesterol-assimilating bacterium Mycobacterium sp. NRRL B-3683, respectively, using nitrosoguanidine (NTG) mutagenesis. Production of AD or ADD from phytosterol by the two mutants...