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Efficient transfer of therapeutic genes into nondividing human cells can be accomplished by inserting the genes into lentiviruses and infecting the cells with the modified viruses. The most developed lentivirus gene transfer systems are based on HIV-1, but because of the widespread HIV epidemic, the use of HIV-based vectors for gene therapy may be associated with a safety risk. In an attempt to find...
Five premature termination mutations and five missense mutations were introduced into the portion of cloned Moloney murine leukemia virus (M-MuLV) DNA encoding the Env cytoplasmic domain. All of the mutant DNAs gave rise to replication-competent virus after transfection of NIH/3T3 cells, but several of the mutant DNAs scored as replication-defective when introduced into Rat2 cells. Cell lines stably...
The location and nature of the HIV-1 packaging signal are largely unknown, despite several genetic and biochemical mutational analyses. In this report we present our attempts to define a minimal HIV-1 packaging signal through the generation of test RNAs containing small blocks of HIV-1 sequences. We constructed RNAs differing in the position and identity of the HIV-1 sequence and the segments of heterologous...
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