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Recent studies have shown that expression levels of different alleles at the same HLA class I locus can vary dramatically, which might have a broad influence on human disease. However, precise quantification of the relative expression level of each HLA allele is challenging, because distinguishing different alleles on the same locus is difficult. Here, we developed a series of allele‐specific, real‐time...
Next generation sequencing (NGS)‐based human leukocyte antigen (HLA) typing was used for ultra large‐scale genotyping of registry donors for the China Marrow Donor Program (CMDP). More than 79,000 samples were subjected to HLA genotyping at 4‐digit allelic level without ambiguities for HLA‐A, ‐B, ‐C, DRB1 and DQB1 loci, with throughput up to 2068 samples per lane in a HiSeq flow cell (eight lanes...
Currently, little information is available for major histocompatibility complex (MHC)‐I that conditions the T‐cell response of marmosets. In this study, 471 clones of MHC‐I cDNA sequences were isolated from 12 marmosets. Twenty full‐length sequences of class I G (Caja‐G) alleles were obtained from these marmosets, 15 of them were novel. Among these 20 Caja‐G alleles, 10 were found in individual animals...
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